期刊
JOURNAL OF BIOCHEMICAL AND MOLECULAR TOXICOLOGY
卷 26, 期 4, 页码 139-146出版社
WILEY-BLACKWELL
DOI: 10.1002/jbt.20420
关键词
Ochratoxin A; Apoptosis; Signaling Pathway; C-Jun NH2 Terminal Kinase; Mitogen-Activated Protein Kinase
资金
- Nature Science Fund of Hebei province [C2006000832]
- Project of Study and Development of Science and Technology of Hebei Province [07276101D-14]
Ochratoxin A (OTA) is nephrotoxic, immunosuppressive, and teratogenic in many species and is a possible human carcinogen. In this study, we investigated the OTA pollution situations of grains in northern China and the signaling pathway that mediated OTA-induced apoptosis in human tubular kidney cells (HKCs). Samples of grains collected from three representative areas were determined by using high-performance liquid chromatography fluorescence method. The effects of OTA on cell apoptosis, caspase-3, Bax, and Bcl-2 expression, and phosphorylation of c-Jun NH2 terminal kinase (JNK) were detected in cultured HKCs via flow cytometry (FCM), Hoechst 33258 staining, and Western blot. It showed that OTA pollution of edible grains was very common in north China. OTA could affect caspase-3, Bax, and Bcl-2 expression and increased cell apoptosis in cultured HKCs. The JNK signalling pathway might play an important role during these cellular events. (C) 2012 Wiley Periodicals, Inc. J Biochem Mol Toxicol 26:139146, 2012; View this article online at . DOI 10.1002/jbt.20420
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