期刊
JOURNAL OF BIOCHEMICAL AND MOLECULAR TOXICOLOGY
卷 26, 期 12, 页码 510-515出版社
WILEY
DOI: 10.1002/jbt.21460
关键词
Latrodectus tredecimguttatus; Egg; Protein; Composition; Toxicity
资金
- National Natural Science Foundation of China [31070700]
- National Basic Research Program or 973 Program of China [2010CB529800]
- Hunan Provincial Natural Science Foundation of China [11JJ2019]
- Specialized Research Foundation for the doctorial Program of Higher Education of China [20094306110003]
Previous work found that high-molecular-weight fractions in the egg extract of Latrodectus tredecimguttatus exhibited strong toxicities. For investigating the possible relationship of proteins in the eggs with the toxic effect, the protein composition of the eggs was analyzed using proteomic strategies and compared with that of the spider's venom. SDS-PAGE showed that the proteins of eggs were primarily distributed in the molecular weight range of higher than 55 kDa as well as around 34 kDa, having high abundance proteins with molecular weights of about 60 kDa and 130 kDa. A total of 157 proteins were identified from the egg extract, which were involved in important cellular functions and processes including catalysis, transport, and metabolism regulation. Comparison indicated that the protein composition of eggs is more complex than that of venom, and there are few similarities between the protein composition of the two materials, demonstrating that the eggs have their own distinct toxic mechanism. (C) 2012 Wiley Periodicals, Inc. J Biochem Mol Toxicol 26: 510-515, 2012; View this article online at wileyonlinelibrary.com. DOI 10.1002/jbt.21460
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