期刊
JOURNAL OF BIOCHEMICAL AND BIOPHYSICAL METHODS
卷 70, 期 6, 页码 823-829出版社
ELSEVIER
DOI: 10.1016/j.jbbm.2007.06.002
关键词
amperometric immunosensor; carcinoembryonic antigen; gold nanoparticle; nano-Au/chitosan composite
A sensitive arnperometric immunosensor for carcinoembryonic antigen (CEA) was prepared. Firstly, a porous nano-structure gold (NG) film was formed on glassy carbon electrode (GCE) by electrochemical reduction of HAuCl4 solution, then nano-Au/Chit composite was immobilized onto the electrode because of its excellent membrane-forming ability, and finally the anti-CEA was adsorbed onto the surface of the bilayer gold nanoparticles to construct an anti -CEA/nano-Au/Chit/NG/GCE immunosensor. The characteristics of the modified electrode at different stages of modification were studied by cyclic voltametry (CV). The gold colloid, chitosan and nano-Au/Chit were characterized by transmission electron microscopy and UV-vis spectroscopy. In addition, the performances of the immunosensor were studied in detail. The resulting immunosensor offers a high-sensitivity (13 10 nA/ng/ml) for the detection of CEA and has good correlation for detection of CEA in the range of 0.2 to 120.0 ng/ rnl with a detection limit of 0.06 ng/ml estimated at a signal-to-noise ratio of 3. The proposed method can detect the CEA through one-step immunoassay and would be valuable for clinical immunoassay. (c) 2007 Published by Elsevier B. V.
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