期刊
JOURNAL OF BIOACTIVE AND COMPATIBLE POLYMERS
卷 28, 期 2, 页码 154-166出版社
SAGE PUBLICATIONS LTD
DOI: 10.1177/0883911512469707
关键词
Poly(epsilon-caprolactone); functional scaffold; heparin; vascular grafts; vascular endothelial growth factor
资金
- National Natural Science Foundation of China (NSFC) project [50830104, 81201207]
- National Outstanding Youth Fund [30725030]
- Tianjin Research Program of Basic Application and Advanced Technology [11JCYBJC09800]
In this study, a heparin-conjugated poly(epsilon-caprolactone) electrospun fiber was constructed to develop a functional scaffold for controlled release of vascular endothelial growth factors. The immobilization of vascular endothelial growth factor was achieved through affinity binding between heparin and vascular endothelial growth factor molecules. The sustained release of vascular endothelial growth factor from the scaffold was followed for up to 15 days. The endothelial cell adhesion and proliferation assay demonstrated that immobilized vascular endothelial growth factor maintained its activity. The blood compatibility of the scaffold was evaluated by activated partial thromboplastin time, platelet adhesion test, and arteriovenous shunt, and the functionalized scaffold showed improved anticoagulation properties. The biocompatibility was evaluated by subcutaneous implantation. Results showed that this vascular endothelial growth factor-releasing scaffold stimulated neovascularization with minimum immunological rejection compared to the unmodified poly(epsilon-caprolactone) scaffold. The present study demonstrated a new strategy of building bioactive scaffolds for the development of small-diameter vascular graft.
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