期刊
JOURNAL OF BIOACTIVE AND COMPATIBLE POLYMERS
卷 26, 期 5, 页码 493-507出版社
SAGE PUBLICATIONS LTD
DOI: 10.1177/0883911511420700
关键词
Carrageenan; adipose derived stem cells; hydrogel; cartilage tissue engineering; Transforming Growth Factor; Controlled release
资金
- European NoE EXPERTISSUES [NMP3-CT-2004-500283]
- European FP7 Project Find and Bind [NMP4-SL-2009-229292]
- Portuguese Foundation for Science and Technology (FCT) [PTDC/FIS/68517/2006, PTDC/QUI/69263/2006, BD/39486/2007]
- Fundação para a Ciência e a Tecnologia [PTDC/QUI/69263/2006, PTDC/FIS/68517/2006] Funding Source: FCT
Tissue engineering (TE) is an emerging field for the regeneration of damaged tissues. The combination of hydrogels with stem cells and growth factors (GFs) has become a promising approach to promote cartilage regeneration. In this study, carrageenan-based hydrogels were used to encapsulate both cells and transforming growth factor-beta 1 (TGF-beta 1). The ATDC5 cell line was encapsulated to determine the cytotoxicity and the influence of polymer concentration on cell viability and proliferation. Human adipose-derived stem cells (hASCs) were encapsulated with TGF-beta 1 in the hydrogel networks to enhance the chondrogenic differentiation of hASCs. Specific cartilage extracellular matrix molecules expression by hASCs were observed after 14 days of cultures of the constructs under different conditions. The kappa-carrageenan was found to be a suitable biomaterial for cell and GF encapsulation. The incorporation of TGF-beta 1 within the carrageenan-based hydrogel enhanced the cartilage differentiation of hASCs. These findings indicate that this new system for cartilage TE is very promising for injectable thermoresponsive formulation applications.
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