期刊
JOURNAL OF BIOACTIVE AND COMPATIBLE POLYMERS
卷 26, 期 4, 页码 363-374出版社
SAGE PUBLICATIONS LTD
DOI: 10.1177/0883911511412553
关键词
polyurethane; adipose-derived stem cells; double-nozzle; low-temperature cell loading; hydrogel; rapid prototyping; tissue regeneration
资金
- National Natural Science Foundation of China/the Research Grants Council of Hong Kong (NSFC/RGC) [50731160625]
- National Natural Science Foundation of China [30970748, 30540060, 30440043, 30600140]
- National High Tech 863 Grant [2009AA043801]
- Tekes the Finnish Funding Agency for Technology and Innovation
- Beijing Municipal Science and Technology Commission [H060920050530]
A tubular polyurethane (PU) sandwich-like, adipose-derived stem cell (ADSC)/gelatin/alginate/fibrin construct was fabricated using a double-nozzle, low-temperature (-20 degrees C) deposition technique. The ADSCs survived the fabrication and cryopreservation stages by incorporating a cryoprotectant (glycerol or dimethyl sulfoxide (DMSO)) in the cell/hydrogel system. With 5% DMSO or 10% glycerol alone in the hydrogel, the cell viabilities were retained (73% and 62%, respectively). The three-dimensional construct was effectively preserved below -80 degrees C for more than 1 week. After the freeze/thaw processes, cell viability and proliferation ability were regained. This strategy has the potential to be widely used in complex organ manufacturing techniques.
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