期刊
JOURNAL OF BIOACTIVE AND COMPATIBLE POLYMERS
卷 24, 期 -, 页码 48-62出版社
SAGE PUBLICATIONS LTD
DOI: 10.1177/0883911509103329
关键词
controlled assembly; high-voltage dispersion technology; microencapsulation; adipose derived stem cells (ADSCs); tissue engineering
资金
- National Natural Science Foundation of China/the Research Grants Council of Hong Kong (NSFC/RGC) [50731160625]
- National Natural Science Foundation of China [50575118, 30540060, 30440043]
- Beijing Municipal Science & Technology Commission [H060920050530]
A cell encapsulation device based on the principle of high-voltage dispersion technology was designed and constructed. The parameters that influenced the formation of cell microcapsules, such as; voltage, push speed, electrode distance, and syringe size were analyzed and optimized. Unlike the traditional cell microencapsulating devices, both of the electrodes were separated from the CaCl2 solution in this device, which simplified the procedure of keeping the operation within a sanitary environment. Integrated adipose derived stem cell (ADSC) microcapsules with smooth surface and consistent diameter distribution were prepared. After 1-week in culture as a microcapsule and 3 days assembled in a construct, the ADSCs maintained a high viability and significant proliferation that established a favorable basis for the controlled assembly of cell microcapsules.
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