期刊
JOURNAL OF BASIC MICROBIOLOGY
卷 52, 期 2, 页码 150-159出版社
WILEY
DOI: 10.1002/jobm.201100134
关键词
Streptomyces miharaensis; Antagonistic bacteria; Fusarium wilt; Filipin III; Biofungicides
类别
资金
- Korea Ministry for Food, Agriculture, Forestry and Fisheries
- Kyung Nong Corp.
A number of bacterial strains were isolated from the internal tissue of Trapa japonica. Of these, strain KPE62302H, which had a 16S rDNA sequence identical to that of Streptomyces miharaensis showed antifungal activity against several plant pathogens. Treatment of seeds with strain KPE62302H induced a significant reduction in the incidence of Fusarium wilt in tomato plants compared with untreated controls. An antifungal substance (FP-1) was purified from the culture extract of strain KPE62302H using C18 flash and Sephadex LH-20 column chromatography and reverse phase HPLC. Extensive spectrometric analysis using MS and NMR identified this as filipin III. FP-1 inhibited the mycelial growth of plant pathogenic fungi such as Alternaria mali, Aspergillus niger, Colletotrichum gloeosporioides, C. orbiculare, Cylindrocarpon destructans, Diaporthe citiri, Fusarium oxysporum at 1-0 mu g ml(-1) and also markedly inhibited the development of Fusarium wilt caused by F. oxysporum f.sp. lycopersici in tomato plants by treatment with 10 mu g ml(-1) under greenhouse conditions. The efficacy of FP-1 against Fusarium wilt was comparable to that of the synthetic fungicide benomyl. An egfp-tagged strain of KPE62302H confirmed its ability to colonize tomato plants.
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