期刊
JOURNAL OF BASIC MICROBIOLOGY
卷 48, 期 4, 页码 315-318出版社
WILEY
DOI: 10.1002/jobm.200800038
关键词
chaperone oligonucleotide microarray; 16S-23S internal transcribed spacer; Kitasatospora
类别
Identification of members of the genus Kitasatospora from soil samples has been introduced to evaluate occurrence of potential natural compound producers in different habitats. The microarray hybridization usually involves PCR amplification of the target DNA. Since PCR might lead to biased amplification, a diagnostic Kitasatospora microarray technique was improved by a protocol lacking PCR amplification prior to hybridization. The described advanced hybridization method used chaperone oligonucleotides for direct co-hybridization with genomic DNA on an oligonuclotide microarray with optical readout.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据