期刊
JOURNAL OF BACTERIOLOGY
卷 194, 期 9, 页码 2297-2306出版社
AMER SOC MICROBIOLOGY
DOI: 10.1128/JB.00119-12
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资金
- National Natural Science Foundation of China [30770049, 30825003]
- State Key Basic Research Development Program of China [2008CB418001]
HetR is the master regulator of heterocyst differentiation in Anabaena sp. strain PCC 7120 and has been found to specifically bind to an inverted-repeat-containing region upstream of hetP, a heterocyst differentiation gene. However, no such inverted-repeat sequence can be found in promoters of other genes in the genome. hetZ is a gene involved in early heterocyst differentiation. As shown with the gfp reporter gene, transcription from P-hetZ was correlated to the expression level of hetR and inhibition by RGSGR, the pentapeptide derived from the C terminus of PatS. As detected by electrophoretic mobility shift assay, a recombinant HetR showed specific binding to the region upstream of hetZ, and the binding was inhibited by RGSGR. Tests of a series of the upstream fragments delimited the HetR-binding site to a 40-bp region that shows similarity to that upstream of hetP. The introduction of substitutions of bases conserved in the two HetR-binding sites showed that at least 12 bases are required for recognition by HetR. Deletion of a 51-bp region containing the HetR-binding site completely eliminated the transcription activity of P-hetZ. Based on the HetR recognition sequence of hetZ, those upstream of hetR and patA are proposed.
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