期刊
JOURNAL OF BACTERIOLOGY
卷 194, 期 17, 页码 4619-4629出版社
AMER SOC MICROBIOLOGY
DOI: 10.1128/JB.00444-12
关键词
-
类别
资金
- NIH [GM093030]
The structure of the Gram-positive flagellum is poorly understood, and Bacillus subtilis encodes three proteins homologous to the flagellar hook protein from Salmonella enterica. Here we generated a modified B. subtilis hook protein that could be fluorescently stained using a cysteine-reactive dye. We used the fluorescently labeled hook to demonstrate that FlgE is the hook structural protein and that FliK regulated hook length. We further demonstrate that two proteins of unknown function, FlhO and FlhP, and the putative hook cap, FlgD, were required for hook assembly, such that when flhO, flhP, or flgD was mutated, hook protein was secreted into the supernatant. All mutants defective in hook completion resulted in homogeneously reduced sigma(D)- dependent gene expression due to the action of the anti-sigma factor FlgM.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据