期刊
JOURNAL OF BACTERIOLOGY
卷 194, 期 1, 页码 122-130出版社
AMER SOC MICROBIOLOGY
DOI: 10.1128/JB.05857-11
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资金
- Ministry of Education, Culture, Sports, Science, and Technology of Japan
- Institution for Fermentation Osaka
- JSPS
Mycoplasma mobile glides on solid surfaces by the repeated binding of leg structures to sialylated oligosaccharide fixed on a solid surface. To obtain information about the propulsion caused by the leg, we made elongated and stiff cells using a detergent. Within 30 min after the cells were treated with 0.1% Tween 60, the cells were elongated from 0.8 mu m to 2.2 mu m in length while maintaining their gliding activity. Fluorescence and electron microscopy showed that a part of the cytoskeletal structure was elongated, while the localization of proteins involved in the gliding was not modified significantly. The elongated cells glided with repeated pivoting around the cellular position of gliding machinery by 10 degrees of amplitude at a frequency of 2 to 3 times per second, suggesting that the propulsion in a line perpendicular to the cell axis can occur with different timings. The pivoting speed decreased as the cell length increased, probably from the load generated by the friction. The torque required to achieve the actual pivoting increased with the cell length without saturation, reaching 54.7 pN nm at 4.3 mu m in cell length.
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