期刊
JOURNAL OF BACTERIOLOGY
卷 193, 期 18, 页码 4736-4748出版社
AMER SOC MICROBIOLOGY
DOI: 10.1128/JB.01533-10
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资金
- Consejo Nacional de Investigaciones Cientificas y Tecnicas (CONICET)
- Departamento de Salud Publica
- Municipalidad de Rosario
- Agencia Nacional de Promocion Cientifica y Tecnoloigica (ANPCyT) Argentina,
- U.S. Public Health [AI070174]
- NSF [0420479]
- Miami University
- Direct For Biological Sciences
- Div Of Biological Infrastructure [0420479] Funding Source: National Science Foundation
We described previously the presence in Acinetobacter baumannii of a novel outer membrane (OM) protein, CarO, which functions as an L-ornithine OM channel and whose loss was concomitant with increased carbapenem resistance among clonally related nosocomial isolates of this opportunistic pathogen. Here, we describe the existence of extensive genetic diversity at the carO gene within the A. baumannii clinical population. The systematic analysis of carO sequences from A. baumannii isolates obtained from public hospitals in Argentina revealed the existence of four highly polymorphic carO variants among them. Sequence polymorphism between the different A. baumannii CarO variants was concentrated in three well-defined protein regions that superimposed mostly to predicted surface-exposed loops. Polymorphism among A. baumannii CarO variants was manifested in differential electrophoretic mobilities, antigenic properties, abilities to form stable oligomeric structures, and L-ornithine influx abilities through the A. baumannii OM under in vivo conditions. Incongruence between the phylogenies of the clinical A. baumannii isolates analyzed and those of the carO variants they harbor suggests the existence of assortative (entire-gene) carO recombinational exchange within the A. baumannii population. Exchange of carO variants possessing differential characteristics mediated by horizontal gene transfer may constitute an A. baumannii population strategy to survive radically changing environmental conditions, such as the leap from inanimate sources to human hosts and vice versa, persistence in a compromised host, and/or survival in health care facilities.
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