期刊
JOURNAL OF BACTERIOLOGY
卷 192, 期 17, 页码 4413-4424出版社
AMER SOC MICROBIOLOGY
DOI: 10.1128/JB.00494-10
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资金
- Ministerio de Educacion, Ciencia y Deporte
- Ministerio de Sanidad, Spain
- Generalitat de Catalunya (Centre de Referencia en Biotecnologia)
- AECI
In this study, we report the identification of genes required for the biosynthesis of the core lipopolysaccharides (LPSs) of two strains of Proteus mirabilis. Since P. mirabilis and Klebsiella pneumoniae share a core LPS carbohydrate backbone extending up to the second outer-core residue, the functions of the common P. mirabilis genes was elucidated by genetic complementation studies using well-defined mutants of K. pneumoniae. The functions of strain-specific outer-core genes were identified by using as surrogate acceptors LPSs from two well-defined K. pneumoniae core LPS mutants. This approach allowed the identification of two new heptosyltransferases (WamA and WamC), a galactosyltransferase (WamB), and an N-acetylglucosaminyltransferase (WamD). In both strains, most of these genes were found in the so-called waa gene cluster, although one common core biosynthetic gene (wabO) was found outside this cluster.
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