期刊
JOURNAL OF BACTERIOLOGY
卷 190, 期 24, 页码 8204-8214出版社
AMER SOC MICROBIOLOGY
DOI: 10.1128/JB.00752-08
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- New Energy and Industrial Technology Development Organization (NEDO)
In previous work, random genome deletion mutants of Corynebacterium glutamicum R were generated using the insertion sequence ( IS) element IS31831 and the Cre/loxP excision system. One of these mutants, C. glutamicum strain RD41, resulting from the deletion of a 10.1-kb genomic region (Delta cgR_1595 through cgR_1604) from the WT strain, showed cell elongation, and several lines appeared on the cell surface ( bamboo shape). The morphological changes were suppressed by overexpression of cgR_1596. Single disruption of cgR_1596 in WT C. glutamicum R resulted in morphological changes similar to those observed in the RD41 strain. CgR_1596 has a predicted secretion signal peptide in the amino-terminal region and a predicted NlpC/P60 domain, which is conserved in cell wall hydrolases, in the carboxyl-terminal region. In C. glutamicum R, CgR_0802, CgR_1596, CgR_2069, and CgR_ 2070 have the NlpC/P60 domain; however, only simultaneous disruption of cgR_1596 and cgR_2070, and not cgR_2070 single disruption, resulted in cell growth delay and more severe morphological changes than disruption of cgR_1596. Transmission electron microscopy revealed multiple septa within individual cells of cgR_1596 single and cgR_1596-cgR_2070 double disruptants. Taken together, these results suggest that cgR_1596 and cgR_2070 are involved in cell separation and cell growth in C. glutamicum.
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