期刊
JOURNAL OF ASSISTED REPRODUCTION AND GENETICS
卷 29, 期 10, 页码 1083-1089出版社
SPRINGER/PLENUM PUBLISHERS
DOI: 10.1007/s10815-012-9831-x
关键词
Oxidative stress; Ovarian stimulation; IVF; IUI; Paraoxonase; Superoxide Dismutases; Interleukin-6; Glutathione Peroxidase; 8-Isoprostane; Fatty acids; Arachidic; Palmitic; Stearic; Oleic; Linoleic & Linolenic
资金
- University of Massachusetts Lowell
We investigated the activities and relevance of a validated panel of antioxidant enzymes, cytokines, specific lipid peroxidation end products and six fatty acids by correlational analyses with peak E-2 levels and pregnancy outcome after ovarian stimulation for IVF or IUI. Blood samples obtained from 15 patients undergoing ovarian stimulation with rFSH or hMG were divided into two groups. Group-1 was baseline blood collected on day-2-3 of women cycle. Group-2 is blood collected at the end of FSH/hMG injection. Serum was collected and stored in liquid nitrogen at -196 A degrees C until analysis. Standard IVF and IUI procedures were followed. The serum levels of Paraoxonase (PON1), Superoxide Dismutases (SOD), Interleukin-6 (IL-6), Glutathione Peroxidase (GPx), 8-Isoprostane, and fatty acids Arachidic, Palmitic, Stearic, Oleic, Linoleic & Linolenic were measured. With the exception of 8-Isoprostane, results showed a positive correlation between baseline and peak levels of E-2 and that of SOD, GPx, PON1, and IL-6. The PON1, IL-6 and SOD were significantly (p < 0.05) higher in pregnant than non-pregnant group. Fatty acid levels at baseline and peak E-2 were not different but pregnancy rates were found to be decreasing with higher palmitic, and stearic acid levels. Ovarian stimulation causes a significant increase in serum PON1, SOD, GPx and IL-6 activity in women undergoing IVF or IUI. The high levels of IL-6, SOD, and PON1 and lower levels of palmitic, and stearic acids in the pregnancy positive group indicate that these oxidative stress and nutritional factors may be used as a predictive marker in controlled ovarian stimulation success.
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