期刊
JOURNAL OF ASSISTED REPRODUCTION AND GENETICS
卷 27, 期 2-3, 页码 93-96出版社
SPRINGER/PLENUM PUBLISHERS
DOI: 10.1007/s10815-010-9385-8
关键词
Built-in microscope incubator; Embryo grade; ICSI; Time-lapse observation
To assess the effects of light from an integrated optical microscope and evaluate the safety of time-lapse observations using a built-in microscope incubator. We prospectively compared the fertilization rate and embryonic morphology after intracytoplasmic sperm injection between embryos cultured with time-lapse observations every 15 min in an incubator with an integrated optical microscope and embryos with intermittent observations (once a day) in conventional incubators. No significant differences were observed in the fertilization rate (57.5% vs. 57.5%) or the rate of excellent-good cleavage embryos (36.0% vs. 36.0%). These results suggest that time-lapse observations using an incubator with an integrated optical microscope may therefore be safely utilized in clinical practice.
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