期刊
JOURNAL OF ASSISTED REPRODUCTION AND GENETICS
卷 27, 期 4, 页码 131-140出版社
SPRINGER/PLENUM PUBLISHERS
DOI: 10.1007/s10815-010-9394-7
关键词
Cryopreservation; Electron microscopy; Oocytes; Slow freezing; Ultrastructure
资金
- Italian National Health Institute
- Italian Ministry of Education, University and Research
To ascertain possible cell damage from cryopreservation, the ultrastructure of human oocytes cryopreserved by slow cooling was assessed. Cryopreservation was performed through two protocols with one-step or two-step propanediol. Fresh control oocytes were examined for comparison. Samples were processed for transmission electron microscopy analysis. By light microscopy, both fresh and frozen-thawed oocytes appeared regularly rounded, with intact zona pellucida, and homogeneous cytoplasm. By electron microscopy observation, organelles were abundant and uniformly dispersed. Mitochondria-smooth endoplasmic reticulum associations appeared regular. However, both the amount and density of cortical granules appeared abnormally reduced in frozen-thawed samples. Slight to moderate vacuolization was also found in the ooplasm of oocytes of both frozen groups. Slow cooling ensures a good overall preservation of human oocytes. However, cytoplasmic vacuolization and cortical granule loss appears associated with cryopreservation, irrespective of the protocol used.
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