期刊
JOURNAL OF ASIAN NATURAL PRODUCTS RESEARCH
卷 15, 期 10, 页码 1073-1079出版社
TAYLOR & FRANCIS LTD
DOI: 10.1080/10286020.2013.812078
关键词
Elaeagnaceae; Elaeagnus pungens; flavonol glycoside; spectroscopic analysis
资金
- Natural Science Foundation of China [31000156]
- National Twelfth Five-Year' Plan for Science & Technology Support of China [2012BAI27B06]
The leaves of Elaeagnus pungens were extracted with 70% ethanol and successively purified by column chromatography. Seven constituents were obtained and characterized, all of which belong to the class of flavonol glycosides. Their structures were elucidated on the basis of spectroscopic methods including 1D/2D NMR and MS analysis techniques. The seven flavonol glycosides were determined as kaempferol 3-O--d-glucopyranosyl-(13)--l-rhamnopyranosyl-(16)-[-l-rhamnopyranosyl(12)]--d-galactopyranoside (1), isorhamnetin 3-O--d-glucopyranosyl-(13)--l-rhamnopyranosyl-(16)--d-galactopyranoside (2), together with five known compounds, respectively. The 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazoliu-m bromide assay in vitro showed that the isolated flavonol glycosides showed no proliferation activity in the asthma airway smooth muscle cells, comparing with solvent as the control group.
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