期刊
JOURNAL OF ARCHAEOLOGICAL SCIENCE
卷 36, 期 8, 页码 1758-1763出版社
ACADEMIC PRESS LTD- ELSEVIER SCIENCE LTD
DOI: 10.1016/j.jas.2009.04.004
关键词
Proteomics; Enamel; Neanderthal; MALDI-TOF-TOF
资金
- Max Planck Gesellschaft
We report here the first results of a method to extract and sequence mature enamel proteins from modern human and Neanderthal tooth enamel. Using MALDI-TOF/TOF mass spectrometry and a combination of direct sequencing and peptide mass mapping we have sequenced a peptide from the tyrosine-rich amelogenin peptide (TRAP) of the X isoform of the amelogenin protein for modern and recent human samples. We also report our results from two Neanderthal enamel samples where we were also able to recover fragments of the TRAP protein, which had a similar sequence to the modern human samples. (C) 2009 Elsevier Ltd. All rights reserved.
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