期刊
JOURNAL OF APPLIED TOXICOLOGY
卷 34, 期 12, 页码 1379-1388出版社
WILEY
DOI: 10.1002/jat.2970
关键词
metabolomics; nuclear magnetic resonance; naphthalene; respiratory toxicity; bronchoalveolar lavage fluid
类别
资金
- National Science Council [NSC97231 4B002016MY2]
- National Health Research Institutes in Taiwan [NHRI-EX99-9915EC, NHRI-EX100-9915EC, NHRI-EX101-9915EC]
Naphthalene is a ubiquitous environmental pollutant to which humans are exposed. Previous studies have demonstrated that naphthalene causes bronchiolar epithelial necrosis in the mouse distal airway, after parenteral administration. In this study, metabolic variations in the bronchoalveolar lavage fluid (BALF) and the lung tissues of naphthalene-treated mice and controls were examined using nuclear magnetic resonance (NMR)-based metabolomics to identify the toxic mechanism. Male ICR mice were treated with naphthalene [0, 50, 100 and 200mgkg(-1), intraperitoneally (i.p.)]. After 24h, BALF and lung tissues were collected and prepared for H-1 and J-resolved (JRES) NMR analysis after principal component analysis (PCA). PCA modeling of p-JRES spectra from the BALF, as well as hydrophilic and hydrophobic lung metabolites, enabled the high-dose group to be discriminated from the control group; increased levels of isopropanol, ethane, and acetone and lower levels of ethanol, acetate, formate, and glycerophosphocholine were detected in the BALF of mice treated with higher doses of naphthalene. Furthermore, increased isopropanol and phosphorylcholine-containing lipid levels and decreased succinate and glutamine levels were discovered in the lungs of naphthalene-exposed mice. These metabolic changes may be related to lipid peroxidation, disruptions of membrane components and imbalanced energy supply, and these results may partially explain the loss of cell membrane integrity in the airway epithelial cells of naphthalene-treated mice. We conclude that NMR-based metabolomic studies on BALF and lung tissues are a powerful tool to understand the mechanisms underlying respiratory toxicity. Copyright (c) 2014 John Wiley & Sons, Ltd. Naphthalene, a ubiquitous environmental pollutant, has been shown to cause bronchiolar epithelial necrosis in the mouse airway. Metabolic variations in the bronchoalveolar lavage fluid and the lung tissues of naphthalene-treated mice and controls were examined using NMR-based metabolomics to identify the toxic mechanism. The metabolic changes may be related to lipid peroxidation, disruptions of membrane components, and imbalanced energy supply, and these results may partially explain the loss of cell membrane integrity in the airway epithelial cells of naphthalene-treated mice.
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