期刊
JOURNAL OF APPLIED TOXICOLOGY
卷 35, 期 4, 页码 407-417出版社
WILEY
DOI: 10.1002/jat.3034
关键词
crown-of-thorns starfish Acanthaster planci; venom; plancitoxin I; A375; S2; cytotoxicity; apoptosis
类别
资金
- National Science Council, Taiwan
- Center of Excellence for Marine Bioenvironment and Biotechnology, National Taiwan Ocean University
This study reports on a cytotoxic toxin derived from the venom of the crown-of-thorns starfish Acanthaster planci (CAV). The protein toxin was isolated through both ion-exchange and gel-filtration chromatography, and characterized by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and mass spectrum analyzes. The CAV was identified as plancitoxin I protein. The mechanistic role of the CAV toxin was explored in human malignant melanoma A375.S2 cell death. The results indicated that after incubation with CAV toxin, cells significantly decreased in A375.S2 cell viability and increased in the lactate dehydrogenase (LDH) level in a dose-dependent manner. The assays indicated that CAV toxin promoted reactive oxygen species (ROS) production, induced nitric oxide (NO) formation, lost mitochondrial membrane potential (m) and induced inter-nucleosomal DNA fragmentation in A375.S2 cells. The molecular cytotoxicity of the CAV toxin was tested through evaluation of the apoptosis/necrosis ratio by double staining with annexin V-FITC and a propidium iodide (PI) assay. The results suggested that CAV toxin induced a cytotoxic effect in A375.S2 cells via the apoptotic procedure, and may be associated with the regulation of the p38 pathways. Copyright (c) 2014 John Wiley & Sons, Ltd. This study reports a cytotoxic toxin derived from the venom of the crown-of-thorns starfish Acanthaster planci (CAV). The CAV was identified as plancitoxin I protein. The results indicated that after incubation with CAV, cells significantly decreased in A375.S2 cell viability. The assays indicated that CAV toxin promoted ROS production, lost mitochondrial membrane potential and induced inter-nucleosomal DNA fragmentation in A375.S2 cells. The results suggested that CAV toxin induced a cytotoxic effect in A375.S2 cells via the apoptotic procedure.
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