期刊
NATURE NEUROSCIENCE
卷 18, 期 6, 页码 917-U373出版社
NATURE PUBLISHING GROUP
DOI: 10.1038/nn.4016
关键词
-
资金
- Enlight Foundation Interdisciplinary Fellowship
- Howard Hughes Medical Institute
- US National Institutes of Health [R01-DC005982, R01-EY022638, R01-DC013070]
- Whitehall Foundation
Intracellular Ca2+ is a widely used neuronal activity indicator. Here we describe a transcriptional reporter of intracellular Ca2+ (TRIC) in Drosophila that uses a binary expression system to report Ca2+-dependent interactions between calmodulin and its target peptide. We found that in vitro assays predicted in vivo properties of TRIC and that TRIC signals in sensory systems depend on neuronal activity. TRIC was able to quantitatively monitor neuronal responses that changed slowly, such as those of neuropeptide F-expressing neurons to sexual deprivation and neuroendocrine pars intercerebralis cells to food and arousal. Furthermore, TRIC-induced expression of a neuronal silencer in nutrient-activated cells enhanced stress resistance, providing a proof of principle that TRIC can be used for circuit manipulation. Thus, TRIC facilitates the monitoring and manipulation of neuronal activity, especially those reflecting slow changes in physiological states that are poorly captured by existing methods. TRIC's modular design should enable optimization and adaptation to other organisms.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据