期刊
JOURNAL OF APPLIED SPECTROSCOPY
卷 76, 期 4, 页码 536-541出版社
SPRINGER
DOI: 10.1007/s10812-009-9227-6
关键词
fluorescence; tryptophan; human serum albumin; denaturation; sodium dodecyl sulfate
类别
资金
- Russian Foundation for Basic Research [07-02-00459]
An analysis of the intrinsic tryptophan fluorescence of human serum albumin (HSA) confirms that the denaturation of HSA by sodium dodecyl sulfate takes place in two stages for different pH levels: the first is the disintegration of globules and the second is the complete unfolding of the amino acid chain of HSA. At pH levels below the isoelectric point (pI 4.7) of HSA, denaturation proceeds through both stages, but when the pH is above pI, denaturation ceases in the first stage.
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