期刊
NATURE NANOTECHNOLOGY
卷 10, 期 8, 页码 696-700出版社
NATURE PUBLISHING GROUP
DOI: 10.1038/NNANO.2015.132
关键词
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资金
- American Heart Association Scientist Development Grant [13SDG14270009]
- National Institutes of Health (NIH) [1DP2 CA186752-01, 1-R01-GM-105646-01-A1, GM33289, HL117138, F32 HL123247-02]
- Lucile Packard CHRI Postdoctoral Award
The sarcomere of muscle is composed of tens of thousands of myosin motors that self-assemble into thick filaments and interact with surrounding actin-based thin filaments in a dense, near-crystalline hexagonal lattice(1). Together, these actin-myosin interactions enable large-scale movement and force generation, two primary attributes of muscle. Research on isolated fibres has provided considerable insight into the collective properties of muscle, but how actin-myosin interactions are coordinated in an ensemble remains poorly understood(2). Here, we show that artificial myosin filaments, engineered using a DNA nanotube scaffold, provide precise control over motor number, type and spacing. Using both dimeric myosin V- and myosin VI-labelled nanotubes, we find that neither myosin density nor spacing has a significant effect on the gliding speed of actin filaments. This observation supports a simple model of myosin ensembles as energy reservoirs that buffer individual stochastic events to bring about smooth, continuous motion. Furthermore, gliding speed increases with cross-bridge compliance, but is limited by Brownian effects. As a first step to reconstituting muscle motility, we demonstrate human beta-cardiac myosin-driven gliding of actin filaments on DNA nanotubes.
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