期刊
NATURE METHODS
卷 12, 期 5, 页码 415-+出版社
NATURE PUBLISHING GROUP
DOI: 10.1038/NMETH.3339
关键词
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资金
- UK Engineering and Physical Research Council [EP/G036004/1, EP/J021849/1, EP/J02175X/1]
- Engineering and Physical Sciences Research Council [EP/K020617/1, EP/J02175X/1, EP/M002306/1, EP/G036004/1, EP/J021849/1, EP/M002187/1] Funding Source: researchfish
- EPSRC [EP/J02175X/1, EP/G036004/1, EP/J021849/1, EP/M002306/1, EP/M002187/1, EP/K020617/1] Funding Source: UKRI
Heterologous gene expression can be a significant burden for cells. Here we describe an in vivo monitor that tracks changes in the capacity of Escherichia coli in real time and can be used to assay the burden imposed by synthetic constructs and their parts. We identify construct designs with reduced burden that predictably outperformed less efficient designs, despite having equivalent output.
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