期刊
NATURE METHODS
卷 12, 期 8, 页码 767-U114出版社
NATURE PUBLISHING GROUP
DOI: 10.1038/NMETH.3453
关键词
-
资金
- NIH [NIDA DA037150, NS076465, T32 HD060600, T32 CA062948]
- German Research Foundation (DFG)
- Irma T. Hirschl and Monique Weill-Caulier Charitable Trusts
- STARR Consortium [I7-A765]
- Vallee Foundation
- WorldQuant Foundation
- EUNICE KENNEDY SHRIVER NATIONAL INSTITUTE OF CHILD HEALTH & HUMAN DEVELOPMENT [T32HD060600] Funding Source: NIH RePORTER
- NATIONAL CANCER INSTITUTE [T32CA062948] Funding Source: NIH RePORTER
- NATIONAL INSTITUTE OF NEUROLOGICAL DISORDERS AND STROKE [R01NS076465] Funding Source: NIH RePORTER
- NATIONAL INSTITUTE ON DRUG ABUSE [R01DA037150] Funding Source: NIH RePORTER
N6-methyladenosine (m6A) is the most abundant modified base in eukaryotic mRNA and has been linked to diverse effects on mRNA fate. Current mapping approaches localize m6A residues to transcript regions 100-200 nt long but cannot identify precise m6A positions on a transcriptome-wide level. Here we developed m6A individual-nucleotide-resolution cross-linking and immunoprecipitation (miCLIP) and used it to demonstrate that antibodies to m6A can induce specific mutational signatures at m6A residues after ultraviolet light-induced antibody-RNA cross-linking and reverse transcription. We found that these antibodies similarly induced mutational signatures at N-6,2'-O-dimethyladenosine (m6Am), a modification found at the first nucleotide of certain mRNAs. Using these signatures, we mapped m6A and m6Am at single-nucleotide resolution in human and mouse mRNA and identified small nucleolar RNAs (snoRNAs) as a new class of m6A-containing non-coding RNAs (ncRNAs).
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据