期刊
NATURE METHODS
卷 12, 期 10, 页码 927-930出版社
NATURE PUBLISHING GROUP
DOI: 10.1038/NMETH.3554
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资金
- US National Institute of Health NIDDK [R01DK101328]
- NHLBI [P01HL053750]
- NHGRI [U54HG007010]
Regulatory regions harbor multiple transcription factor (TF) recognition sites; however, the contribution of individual sites to regulatory function remains challenging to define. We describe an approach that exploits the error-prone nature of genome editing-induced double-strand break repair to map functional elements within regulatory DNA at nucleotide resolution. We demonstrate the approach on a human erythroid enhancer, revealing single TF recognition sites that gate the majority of downstream regulatory function.
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