期刊
NATURE METHODS
卷 12, 期 7, 页码 641-+出版社
NATURE PUBLISHING GROUP
DOI: 10.1038/NMETH.3402
关键词
-
资金
- Ministere de l'Enseignement Superieur et de la Recherche [ANR-10-INBS-04]
- Regional Council of Aquitaine
- Singapore National Research Foundation (NRF)
- Mechanobiology Institute through an NRF grant
Single-objective selective-plane illumination microscopy (soSPIM) is achieved with micromirrored cavities combined with a laser beam-steering unit installed on a standard inverted microscope. The illumination and detection are done through the same objective. soSPIM can be used with standard sample preparations and features high background rejection and efficient photon collection, allowing for 3D single-molecule-based super-resolution imaging of whole cells or cell aggregates. Using larger mirrors enabled us to broaden the capabilities of our system to image Drosophila embryos.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据