期刊
NATURE METHODS
卷 12, 期 5, 页码 404-+出版社
NATURE PUBLISHING GROUP
DOI: 10.1038/NMETH.3323
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资金
- SystemsX.ch
- Swiss national initiative for Systems Biology
- Finnish TEKES FiDiPro
- Hungarian National Brain Research Programme (MTA-SE-NAP B-BIOMAG)
Uneven illumination affects every image acquired by a microscope. It is often overlooked, but it can introduce considerable bias to image measurements. The most reliable correction methods require special reference images, and retrospective alternatives do not fully model the correction process. Our approach overcomes these issues for most optical microscopy applications without the need for reference images.
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