期刊
NATURE METHODS
卷 12, 期 12, 页码 1179-+出版社
NATURE PUBLISHING GROUP
DOI: 10.1038/nmeth.3603
关键词
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资金
- Proteins@Work, a program of the Netherlands Proteomics Centre
- Netherlands Organisation for Scientific Research (NWO), National Roadmap Large-scale Research Facilities of the Netherlands [184.032.201]
We describe an integrated workflow that robustly identifies cross-links from endogenous protein complexes in human cellular lysates. Our approach is based on the application of mass spectrometry (MS)-cleavable cross-linkers, sequential collision-induced dissociation (CID)-tandem MS (MS/MS) and electron-transfer dissociation (ETD)-MS/MS acquisitions, and a dedicated search engine, XlinkX, which allows rapid cross-link identification against a complete human proteome database. This approach allowed us to detect 2,179 unique cross-links (1,665 intraprotein cross-links at a 5% false discovery rate (FDR) and 514 interprotein cross-links at 1% FDR) in HeLa cell lysates. We validated the confidence of our cross-linking results by using a target-decoy strategy and mapping the observed cross-link distances onto existing high-resolution structures. Our data provided new structural information about many protein assemblies and captured dynamic interactions of the ribosome in contact with different elongation factors.
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