期刊
NATURE METHODS
卷 13, 期 1, 页码 87-+出版社
NATURE PUBLISHING GROUP
DOI: 10.1038/NMETH.3629
关键词
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资金
- NIH from Eunice Kennedy Shriver National Institute of Child Health and Human Development [5R24HD000836]
- NIH [5DP1MH099906-03]
- National Science Foundation [PHY-0952766]
- EUNICE KENNEDY SHRIVER NATIONAL INSTITUTE OF CHILD HEALTH & HUMAN DEVELOPMENT [R24HD000836] Funding Source: NIH RePORTER
- NATIONAL INSTITUTE OF GENERAL MEDICAL SCIENCES [T32GM007598] Funding Source: NIH RePORTER
- NATIONAL INSTITUTE OF MENTAL HEALTH [DP1MH099906] Funding Source: NIH RePORTER
The diverse progenitors that give rise to the human neocortex have been difficult to characterize because progenitors, particularly radial glia (RG), are rare and are defined by a combination of intracellular markers, position and morphology. To circumvent these problems, we developed Fixed and Recovered Intact Single-cell RNA (FRISCR), a method for profiling the transcriptomes of individual fixed, stained and sorted cells. Using FRISCR, we profiled primary human RG that constitute only 1% of the midgestation cortex and classified them as ventricular zone-enriched RG (vRG) that express ANXA1 and CRYAB, and outer subventricular zone-localized RG (oRG) that express HOPX. Our study identified vRG and oRG markers and molecular profiles, an essential step for understanding human neocortical progenitor development. FRISCR allows targeted single-cell profiling of any tissues that lack live-cell markers.
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