期刊
JOURNAL OF APPLIED POLYMER SCIENCE
卷 124, 期 3, 页码 2171-2178出版社
WILEY
DOI: 10.1002/app.35239
关键词
pectin sulfate; sulfating reagent; trisulfonated sodium amine; anticoagulant activity
资金
- National Natural Science Foundation of China [50503019]
- Natural Science Foundation of Hubei Province [2008CDB282]
- Doctor Subject Foundation of the Ministry of Education of China [200804971074]
- Wuhan Science and Technology Development [201060623262]
- Health Department of Hubei Province [JX4B54]
- Wuhan Academic Leaders Program [200851430480]
- Independent Innovation Research Foundation of Wuhan University of Technology [2010-IV-070]
In this article, we describe the first use of trisulfonated sodium amine as a sulfating reagent for preparing pectin sulfate in aqueous solution. The main reaction parameters that were expected to affect the degree of substitution (DS) were studied. The optimal reaction conditions for the synthesis of pectin sulfate were found to be as follows: the pH of the reaction medium, the reaction temperature, the reaction time, and the ratio of the mole of sodium nitrite (nNaNO2) to the mass of pectin (mpectin) were 6, 60 degrees C, 12 h, and 2.5/190 mol/g, respectively. Pectin and pectin sulfate were characterized by Fourier transform infrared (FTIR) spectroscopy and 13C-NMR. The FTIR spectra showed the characteristic absorptions of sulfate ester bonds at 1264 and 830 cm-1. Furthermore, the anticoagulant activity of pectin sulfates with different DSs, concentrations, and molecular weights were investigated with respect to the activated partial thromboplastin time (APTT), thrombin time (TT) and prothombin time. The clotting assay indicated that the pectin sulfate prolonged APTT and TT through inhibition of the activity of antithrombin. These results suggest that the introduction of sulfate groups into the pectin structure improved its anticoagulant activity. (C) 2011 Wiley Periodicals, Inc. J Appl Polym Sci, 2012
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