期刊
JOURNAL OF APPLIED POLYMER SCIENCE
卷 122, 期 3, 页码 1847-1856出版社
WILEY-BLACKWELL
DOI: 10.1002/app.34263
关键词
proteins; templates; hydrogels; molecular imprinting; modeling
资金
- Fuzhou University [0460022305]
- MOE, PRC [2002-123, 02041]
The rebinding behavior of protein-imprinted hydrogel is different from classical small molecularly imprinted polymer especially in protein aggregating state and interaction changeability between protein and imprinted sites. BSA-imprinted calcium alginate-hydroxy ethyl cellulose microspheres were prepared, and the rebinding behavior was studied. An imprinting induce-fit model'' was proposed to describe the rebinding property of protein-imprinted hydrogel. Three kinds of different interacting forms between protein and imprinted hydrogel were discovered by Scatchard analysis. Slogistic fit analysis of rebinding rate coefficient was carried out, and the imprinted hydrogel was found capable of promoting rebinding through induce-fit behavior. Higher imprinting efficiency was found in microsphere samples with lower crosslinking density. Rebinding regression equation was established, and the rebinding quantity was computable with parameters including BSA aggregate concentration [P(n)], dissociating rate (k(1)), and single molecule rebinding rate (k(2)). The experimental and calculated rebinding concentration were compared, and errors between -9.43% and 5.59% were found. (C) 2011 Wiley Periodicals, Inc. J Appl Polym Sci 122: 1847-1856, 2011
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据