期刊
NATURE MATERIALS
卷 14, 期 12, 页码 1269-1277出版社
NATURE PUBLISHING GROUP
DOI: 10.1038/NMAT4407
关键词
-
类别
资金
- Materials Research Science and Engineering Center (MRSEC) at Harvard University [DMR-1420570]
- NIH [R37 DE013033]
- Belgian American Educational Foundation
- NSF Graduate Research Fellowship
- Einstein Visiting Fellowship
- Einstein Foundation Berlin through the Charite-Universitatsmedizin Berlin, Berlin-Brandenburg School for Regenerative Therapies [GSC 203]
- Harvard College Research Program
- Harvard College PRISE
- Herchel-Smith Family Fund Fellowship
- Pechet Family Fund Fellowship
The effectiveness of stem cell therapies has been hampered by cell death and limited control over fate. These problems can be partially circumvented by using macroporous biomaterials that improve the survival of transplanted stem cells and provide molecular cues to direct cell phenotype. Stem cell behaviour can also be controlled in vitro by manipulating the elasticity of both porous and non-porous materials, yet translation to therapeutic processes in vivo remains elusive. Here, by developing injectable, void-forming hydrogels that decouple pore formation from elasticity, we show that mesenchymal stem cell (MSC) osteogenesis in vitro, and cell deployment in vitro and in vivo, can be controlled by modifying, respectively, the hydrogel's elastic modulus or its chemistry. When the hydrogels were used to transplant MSCs, the hydrogel's elasticity regulated bone regeneration, with optimal bone formation at 60 kPa. Our findings show that biophysical cues can be harnessed to direct therapeutic stem cell behaviours in situ.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据