Selecting the correct exercise intensity for unbiased comparisons of thermoregulatory responses between groups of different mass and surface area

We assessed whether comparisons of thermoregulatory responses between groups unmatched for body mass and surface area (BSA) should be performed using a metabolic heat production (H-prod) in Watts or Watts per kilogram for changes in rectal temperature (Delta T-re), and an evaporative heat balance requirement (E-req) in Watts or Watts per square meter for local sweat rates (LSR). Two groups with vastly different mass and BSA [large (LG): 91.5 +/- 6.8 kg, 2.12 +/- 0.09 m(2), n = 8; small (SM): 67.6 +/- 5.6 kg, 1.80 +/- 0.09 m(2), n = 8; P < 0.001], but matched for heat acclimation status, sex, age, and with the same onset threshold esophageal temperatures (LG: +0.37 +/- 0.12 degrees C; SM: + 0.41 +/- 0.17 degrees C; P = 0.364) and thermosensitivities (LG: 1.02 +/- 0.54, SM: 1.00 +/- 0.38 mg cm(-2) min(-1).degrees C-1; P = 0.918) for sweating, cycled for 60 min in 25 degrees C at different levels of H-prod (500 W, 600 W, 6.5 W/kg, 9.0 W/kg) and E-req (340 W, 400 W, 165 W/m(2), 190 W/m(2)). Delta T-re was different between groups at a H-prod of 500 W (LG: 0.52 +/- 0.15 degrees C, SM: 0.92 +/- 0.24 degrees C; P < 0.001) and 600 W (LG: 0.78 +/- 0.19 degrees C, SM: 1.14 +/- 0.24 degrees C; P = 0.007), but similar at 6.5 W/kg (LG: 0.79 +/- 0.21 degrees C, SM: 0.85 +/- 0.14 degrees C; P = 0.433) and 9.0 W/kg (LG: 1.02 +/- 0.22 degrees C, SM: 1.14 +/- 0.24 degrees C; P = 0.303). Furthermore, Delta T-re was the same at 9.0 W/kg in a 35 C environment (LG: 1.12 +/- 0.30 degrees C, SM: 1.14 +/- 0.25 degrees C) as at 25 C (P > 0.230). End-exercise LSR was different at E-req of 400 W (LG: 0.41 +/- 0.18, SM: 0.57 +/- 0.13 mg cm(-2) min(-1); P = 0.043) with a trend toward higher LSR in SM at 340 W (LG: 0.28 +/- 0.06, SM: 0.37 +/- 0.15 mg cm(-2) min(-1); P = 0.057), but similar at 165 W/m(2) (LG: 0.28 +/- 0.06, SM: 0.28 +/- 0.12 mg cm(-2) min(-1); P = 0.988) and 190 W/m(2) (LG: 0.41 +/- 0.18, SM: 0.37 +/- 0.15 mg.cm(-2) min(-1); P = 0.902). In conclusion, when comparing groups unmatched for mass and BSA, future experiments can avoid systematic differences in Delta T-re and LSR by using a fixed H-prod in Watts per kilogram and E-req in Watts per square meter, respectively.