期刊
JOURNAL OF APPLIED PHYSIOLOGY
卷 113, 期 11, 页码 1772-1783出版社
AMER PHYSIOLOGICAL SOC
DOI: 10.1152/japplphysiol.00175.2012
关键词
arrhythmia; myocardial infarction; ion channels; sarcoplasmic reticulum
资金
- National Heart, Lung, and Blood Institute [HL086700, HL063043, HL089836]
Bonilla IM, Belevych AE, Sridhar A, Nishijima Y, Ho H, He Q, Kukielka M, Terentyev D, Terentyeva R, Liu B, Long VP, Gyorke S, Carnes CA, Billman GE. Endurance exercise training normalizes repolarization and calcium-handling abnormalities, preventing ventricular fibrillation in a model of sudden cardiac death. J Appl Physiol 113: 1772-1783, 2012. First published October 4, 2012; doi:10.1152/japplphysiol.00175.2012.-The risk of sudden cardiac death is increased following myocardial infarction. Exercise training reduces arrhythmia susceptibility, but the mechanism is unknown. We used a canine model of sudden cardiac death (healed infarction, with ventricular tachyarrhythmias induced by an exercise plus ischemia test, VF+); we previously reported that endurance exercise training was antiarrhythmic in this model (Billman GE. Am J Physiol Heart Circ Physiol 297: H1171-H1193, 2009). A total of 41 VF+ animals were studied, after random assignment to 10 wk of endurance exercise training (EET; n = 21) or a matched sedentary period (n = 20). Following (>1 wk) the final attempted arrhythmia induction, isolated myocytes were used to test the hypotheses that the endurance exercise-induced antiarrhythmic effects resulted from normalization of cellular electrophysiology and/or normalization of calcium handling. EET prevented VF and shortened in vivo repolarization (P < 0.05). EET normalized action potential duration and variability compared with the sedentary group. EET resulted in a further decrement in transient outward current compared with the sedentary VF+ group (P < 0.05). Sedentary VF+ dogs had a significant reduction in repolarizing K+ current, which was restored by exercise training (P < 0.05). Compared with controls, myocytes from the sedentary VF+ group displayed calcium alternans, increased calcium spark frequency, and increased phosphorylation of S2814 on ryanodine receptor 2. These abnormalities in intracellular calcium handling were attenuated by exercise training (P < 0.05). Exercise training prevented ischemically induced VF, in association with a combination of beneficial effects on cellular electrophysiology and calcium handling.
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