期刊
JOURNAL OF APPLIED PHYSIOLOGY
卷 105, 期 3, 页码 902-906出版社
AMER PHYSIOLOGICAL SOC
DOI: 10.1152/japplphysiol.90558.2008
关键词
contractile protein breakdown; microdialysis; muscle atrophy; spaceflight simulation; 3-methylhistidine
资金
- National Aeronautics and Space Administration
- Swedish Council of Sports
- Centre of Gender Medicine
- Swedish National Space Board
- Loo and Hans Osterman Foundation
- National Institute on Aging [AG-020532]
- NATIONAL INSTITUTE ON AGING [R01AG020532] Funding Source: NIH RePORTER
Skeletal muscle atrophy is evident after muscle disuse, unloading, or spaceflight and results from decreased protein content as a consequence of decreased protein synthesis, increased protein breakdown or both. At this time, there are essentially no human data describing proteolysis in skeletal muscle undergoing atrophy on Earth or in space, primarily due to lack of valid and accurate methodology. This particular study aimed at assessing the effects of short-term unloading on the muscle contractile proteolysis rate. Eight men were subjected to 72-h unilateral lower limb suspension (ULLS) and intramuscular interstitial levels of the naturally occurring proteolytic tracer 3-methylhistidine (3MH) were measured by means of microdialysis before and on completion of this intervention. The 3MH concentration following 72-h ULLS (2.01 +/- 0.22 nmol/ml) was 44% higher (P < 0.05) than before ULLS (1.56 +/- 0.20 nmol/ml). The present experimental model and the employed method determining 3MH in microdialysates present a promising tool for monitoring skeletal muscle proteolysis or metabolism of specific muscles during conditions resulting in atrophy caused by, e. g., disuse and real or simulated microgravity. This study provides evidence that the atrophic processes are evoked rapidly and within 72 h of unloading and suggests that countermeasures should be employed in the early stages of space missions to offset or prevent muscle loss during the period when the rate of muscle atrophy is the highest.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据