期刊
NATURE CHEMISTRY
卷 7, 期 2, 页码 160-165出版社
NATURE PUBLISHING GROUP
DOI: 10.1038/NCHEM.2142
关键词
-
资金
- European Research Council (ERC) [246812]
- Netherlands Organization for Scientific Research (NWO, VICI grant) [700.10.44]
- Marie Curie Intra-European Fellowship [300519]
- Dutch Ministry of Education, Culture and Science (Gravity programme) [024.001.035]
Life is sustained by complex systems operating far from equilibrium and consisting of a multitude of enzymatic reaction networks. The operating principles of biology's regulatory networks are known, but the in vitro assembly of out-of-equilibrium enzymatic reaction networks has proved challenging, limiting the development of synthetic systems showing autonomous behaviour. Here, we present a strategy for the rational design of programmable functional reaction networks that exhibit dynamic behaviour. We demonstrate that a network built around autoactivation and delayed negative feedback of the enzyme trypsin is capable of producing sustained oscillating concentrations of active trypsin for over 65 h. Other functions, such as amplification, analog-to-digital conversion and periodic control over equilibrium systems, are obtained by linking multiple network modules in microfluidic flow reactors. The methodology developed here provides a general framework to construct dissipative, tunable and robust (bio) chemical reaction networks.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据