期刊
NATURE CHEMISTRY
卷 7, 期 10, 页码 802-809出版社
NATURE PUBLISHING GROUP
DOI: 10.1038/NCHEM.2344
关键词
-
资金
- European Research Council
- Biotechnology and Biological Sciences Research Council
- Wellcome Trust
- Newman Foundation
- Winston Churchill Foundation
- Elan Pharmaceuticals
- Medical Research Council [G1002272]
- Biotechnology and Biological Sciences Research Council [BB/J002119/1] Funding Source: researchfish
- Medical Research Council [G1002272] Funding Source: researchfish
- BBSRC [BB/J002119/1] Funding Source: UKRI
- MRC [G1002272] Funding Source: UKRI
The study of biomolecular interactions is central to an understanding of function, malfunction and therapeutic modulation of biological systems, yet often involves a compromise between sensitivity and accuracy. Many conventional analytical steps and the procedures required to facilitate sensitive detection, such as the incorporation of chemical labels, are prone to perturb the complexes under observation. Here we present a 'latent' analysis approach that uses chemical and microfluidic tools to reveal, through highly sensitive detection of a labelled system, the behaviour of the physiologically relevant unlabelled system. We implement this strategy in a native microfluidic diffusional sizing platform, allowing us to achieve detection sensitivity at the attomole level, determine the hydrodynamic radii of biomolecules that vary by over three orders of magnitude in molecular weight, and study heterogeneous mixtures. We illustrate these key advantages by characterizing a complex of an antibody domain in the solution phase and under physiologically relevant conditions.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据