期刊
NATURE CELL BIOLOGY
卷 17, 期 12, 页码 1536-1545出版社
NATURE PUBLISHING GROUP
DOI: 10.1038/ncb3263
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资金
- NIH Office of Research Infrastructure Programs [P40 OD010440]
- Netherlands Organisation for Scientific Research (NWO) via a Vici, an NWO-Groot
- ALW Open Program grant, via the STW research programme Nanoscopy
- FOM programme 'Barriers in the Brain
- NanoNextNL of the Government of the Netherlands
- NIH [GM50718]
Intracellular transport depends on cooperation between distinct motor proteins. Two anterograde intraflagellar transport (IFT) motors, heterotrimeric kinesin-Il and homodimeric OSM-3, cooperate to move cargo along Caenorhabditis elegans cilia. Here, using quantitative fluorescence microscopy, with single-molecule sensitivity, of IFT in living strains containing single-copy transgenes encoding fluorescent IFT proteins, we show that kinesin-II transports IFT trains through the ciliary base and transition zone to a `handover zone' on the proximal axoneme. There, OSM-3 gradually replaces kinesin-II, yielding velocity profiles inconsistent with in vitro motility assays, and then drives transport to the ciliary tip. Dissociated kinesin-Il motors undergo rapid turnaround and recycling to the ciliary base, whereas OSM-3 is recycled mainly to the handover zone. This reveals a functional differentiation in which the slower, less processive kinesin-Il imports IFT trains into the cilium and OSM-3 drives their long-range transport, thereby optimizing cargo delivery.
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