期刊
NATURE BIOTECHNOLOGY
卷 33, 期 2, 页码 175-178出版社
NATURE PUBLISHING GROUP
DOI: 10.1038/nbt.3127
关键词
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资金
- California Institute of Regenerative Medicine [RB3-02161]
- Nesvig Foundation
- Beckman Research Institute Excellence Award
- China Scholarship Council
- National Natural Science Foundation of China
- Zhejiang Provincial Natural Science Foundation of China
- Zhejiang Provincial Major Science and Technology Project
The utility of CRISPR-Cas9 and TALENs for genome editing may be compromised by their off-target activity. We show that integrase-defective lentiviral vectors (IDLVs) can detect such off-target cleavage with a frequency as low as 1%. In the case of Cas9, we find frequent off-target sites with a one-base bulge or up to 13 mismatches between the single guide RNA (sgRNA) and its genomic target; which refines sgRNA design.
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