期刊
NATURE BIOTECHNOLOGY
卷 34, 期 2, 页码 199-+出版社
NATURE PUBLISHING GROUP
DOI: 10.1038/nbt.3445
关键词
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资金
- Swedish Research Council
- Swedish Foundation for Strategic Research [FFL4]
- McKnight Scholar Award
- Arnold and Mabel Beckman Foundation Young Investigator Award
- Deutsche Forschungsgemeinschaft (DFG) [EXC 307]
- German Federal Ministry of Education and Research (BMBF
- BCCN Tubingen) [FKZ 01GQ1002]
- [DP1EY023176]
- [P30EY002520]
- [T32EY07001]
- [DP1OD008301]
- [R01MH103108]
- [F30MH095440]
- [T32GM007330]
- [T32EB006350]
- [R01NS062829]
Despite the importance of the mammalian neocortex for complex cognitive processes, we still lack a comprehensive description of its cellular components. To improve the classification of neuronal cell types and the functional characterization of single neurons, we present Patch-seq, a method that combines whole-cell electrophysiological patch-clamp recordings, single-cell RNA-sequencing and morphological characterization. Following electrophysiological characterization, cell contents are aspirated through the patch-clamp pipette and prepared for RNA-sequencing. Using this approach, we generate electrophysiological and molecular profiles of 58 neocortical cells and show that gene expression patterns can be used to infer the morphological and physiological properties such as axonal arborization and action potential amplitude of individual neurons. Our results shed light on the molecular underpinnings of neuronal diversity and suggest that Patch-seq can facilitate the classification of cell types in the nervous system.
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