Fe doped ZnO thin film for mediator-less biosensing application

Fe doped ZnO (FZO) thin film is prepared by pulsed laser deposition for its application as mediator-less biosensing matrix. Fe doping introduces redox centre in ZnO along with shallow donor level and promotes the electron transfer capability due to substitution of Fe at Zn sites. Glucose oxidase (GOx), chosen as model enzyme, was immobilized on surface of the prepared matrix. Cyclic voltammetry and photometric assay show that the developed bio-electrode, GOx/FZO/indiurn tin oxide/Glass is sensitive to glucose concentration with enhanced response (0.2 mu A mM(-1) cm(-2)) and low K-m (3.01 mM). The results show promising application of Fe doped ZnO thin film as an attractive matrix for mediator-less biosensing. (C) 2012 American Institute of Physics. [http://dx.doi.org/10.1063/1.4714670]