期刊
JOURNAL OF APPLIED PHYCOLOGY
卷 25, 期 1, 页码 337-347出版社
SPRINGER
DOI: 10.1007/s10811-012-9868-3
关键词
AFLP; DNA extraction; DNA purification; Fragment analysis; Saccharina; Phaeophyta
资金
- Ministry of Education, Science, Sports and Culture, Japan [21241055]
- Environment Research and Technology Development Fund (S9) by the Ministry of the Environment, Japan
- Grants-in-Aid for Scientific Research [22580192, 21241055] Funding Source: KAKEN
Deoxyribonucleic acid (DNA) fragment analysis can become an effective tool to study genetic differences between species and individuals on saccharinan kelp from which the little genetic diversity has been reported. Here, extraction methods of DNA suitable for use in analysis with a capillary sequencer is examined on Saccharina japonica var. diabolica which contains abundant polysaccharide. When amplified fragment length polymorphism was performed using genomic DNA extracted by seven different methods: (1) commercial kit, (2) original cetyl trimethylammonium bromide (CTAB) method, (3)-(5) three types of modified CTAB method, (6) modified sodium dodecyl sulfate (SDS) method, (7) combination of CTAB method and SDS method, a high reproducible peak that was suitable for analysis was noticeable in the electropherogram in the experiment with the last combination method (7). It is considered that the pretreatment washing of polysaccharide and the subsequent purification for protein and ribonucleic acid in SDS method and for polysaccharide in CTAB method are effective to obtain the high-purity DNA.
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