期刊
JOURNAL OF APPLIED PHYCOLOGY
卷 24, 期 2, 页码 163-171出版社
SPRINGER
DOI: 10.1007/s10811-011-9664-5
关键词
2-DE; Kelp; Protein profiles; Ecklonia cava; Phaeophyta; Temperature stress
资金
- Wakayama Prefecture Collaboration of Regional Entities for the Advancement of Technological Excellence of Japan Science and Technology Agency (JST)
- Ministry of Education, Science, Sports and Culture, Japan
- Grants-in-Aid for Scientific Research [22580192, 21241055] Funding Source: KAKEN
Proteomic profiling on Ecklonia cava Kjellman grown under various seawater temperatures was conducted to search for biomarkers that were useful to evaluate the health of the colonies and formulate actions for the maintenance of marine forests. In the cultivated strains, protein expression was not significantly changed when the cultivation temperature was lowered from 15A degrees C (control) to 10A degrees C. On the contrary, it was markedly changed, i.e., photosynthesis-related proteins were up-regulated and metabolic enzymes were down-regulated, when the temperature was heightened to 20A degrees C. With the cultivation at 30A degrees C, 25 spots within 27 spots expressed at this temperature peculiarly could be identified and classified into ten proteins. Of the distinctive 27 spots at 30A degrees C, 20 spots were detected in the wild strains cultured at the same temperature for a brief time. It is presumed that the proteins including vanadium-dependent bromoperoxidase are heat stress-induced proteins.
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