期刊
JOURNAL OF APPLIED PHYCOLOGY
卷 24, 期 5, 页码 1203-1208出版社
SPRINGER
DOI: 10.1007/s10811-011-9761-5
关键词
Amylolytic enzymes; Biofuels; Chlorella vulgaris; Microalgae; Perchloric acid; Starch hydrolysis
资金
- Fundacao para a Ciencia e a Tecnologia (Portugal) [SFRH/BD/44724/2008, SFRH/BPD/44935/2008]
- INNOVALGAE [FCT PTDC/AAC-AMB/108511/2008]
- ALGANOL
- Fundação para a Ciência e a Tecnologia [SFRH/BD/44724/2008, SFRH/BPD/44935/2008] Funding Source: FCT
Different methods for estimating starch in Chlorella vulgaris were compared with the view of establishing a procedure suitable for rapid and accurate determination of starch content in this microalgal species. A close agreement was observed between methods that use perchloric acid and enzymatic methods that use alpha-amylase and amyloglucosidase to hydrolyze the starch of microalgae grown under different nitrogen culture conditions. Starch values obtained by these methods were significantly higher than those estimated by using hydrochloric acid as solubilizing and hydrolyzing agent. The enzymatic method (EM1) proved to be the most rapid and precise method for microalgal starch quantification. Furthermore, the evaluation of resistant starch by enzymatic methods assayed in nitrogen-sufficient and nitrogen-starved cells showed that no formation of this type of starch occurred in microalgae, meaning that this should not interfere with starch content determinations.
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