期刊
JOURNAL OF APPLIED ORAL SCIENCE
卷 18, 期 1, 页码 50-58出版社
UNIV SAO PAULO FAC ODONTOLOGIA BAURU
DOI: 10.1590/S1678-77572010000100010
关键词
Chlorhexidine; Odontoblasts; Cytotoxicity; Cell viability; Protein synthesis
资金
- Conselho Nacional de Desenvolvimento Cientifico e Tecnologico/CNPq [476137/2006-3, 301029/2007-5]
- Coordenacao de Aperfeicoamento de Pessoal de Nivel Superior/CAPES
Chlorhexidine gluconate (CHX) is recommended for a number of clinical procedures and it has been pointed out as a potential cavity cleanser to be applied before adhesive restoration of dental cavities. Objective: As CHX may diffuse through the dentinal tubules to reach a monolayer of odontoblasts that underlies the dentin substrate, this study evaluated the cytotoxic effects of different concentrations of CHX on cultured odontoblast-like cells (MDPC-23). Material and Methods: Cells were cultured and exposed to CHX solutions at concentrations of 0.06%, 0.12%, 0.2%, 1% and 2%. Pure culture medium (alpha-MEM) and 3% hydrogen peroxide were used as negative and positive control, respectively. After exposing the cultured cells to the controls and CHX solutions for 60 s, 2 h or 60 s with a 24-h recovery period, cell metabolism (MTT assay) and total protein concentration were evaluated. Cell morphology was assessed under scanning electron microscopy. CHX had a dose-dependent toxic effect on the MDPC-23 cells. Results: Statistically significant difference was observed when the cells were exposed to CHX in all periods (p<0.05). Significant difference was also determined for all CHX concentrations (p<0.05). The 60-s exposure time was the least cytotoxic (p<0.05), while exposure to CHX for 60 s with a 24-h recovery period was the most toxic to the cells (p<0.05). Conclusion: Regardless of the exposure time, all CHX concentrations had a high direct cytotoxic effect to cultured MDPC-23 cells.
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