期刊
JOURNAL OF APPLIED MICROBIOLOGY
卷 111, 期 2, 页码 467-473出版社
WILEY
DOI: 10.1111/j.1365-2672.2011.05067.x
关键词
bacterial culture; bifidobacteria; elderly Irish; lactobacilli; sequencing
资金
- Government of Ireland's Department of Agriculture Fisheries and Food
- Health Research Board, through the Food and Health Initiative
Aims: The aim of this investigation was to establish the degree of correlation between measurements from culture-dependent microbiological techniques and from next generation sequencing technologies. Methods and Results: Data generated by both techniques were collected from faecal samples from 185 elderly Irish people involved in the ongoing ELDER-MET study (http://eldermet.ucc.ie). The results for three groups of intestinal bacteria were compared. Bifidobacterium sp., Lactobacillus sp. and Enterobacteriaceae were enumerated on selective media through culture-dependent techniques, whereas proportions of these bacteria were determined through sequencing technology against the background of other bacteria. The Spearman's rank correlation coefficient determined a good correlation between results from culture-dependent microbiology and culture-independent techniques for all three bacterial groups assessed (correlation coefficients for Bifidobacterium sp., Lactobacillus sp. and Enterobacteriaceae were 0.380, 0.366 and 0.437, respectively). Conclusion: Correlation between the two methods implies that a single method is capable of profiling intestinal Bifidobacterium, Lactobacillus and Enterobacteriaceae populations. However, both methods have advantages that justify their use in tandem. Significance and Impact of the Study: This is the first extensive study to compare bacterial counts from culture-dependent microbiological techniques and from next generation sequencing technologies.
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