期刊
JOURNAL OF APPLIED MICROBIOLOGY
卷 107, 期 6, 页码 1924-1934出版社
WILEY
DOI: 10.1111/j.1365-2672.2009.04376.x
关键词
PCR-DGGE; rumen bacterial diversity; ruminal fermentation parameters
资金
- Alberta Livestock Industry Development Fund [2007F041]
- Alberta Agriculture Research Institute
- Alberta Bovine Genomics Program
Aims: To investigate, using culture-independent methods, whether the ruminal bacterial structure, population and fermentation parameters differed between sampling locations and time. Methods and Results: The detectable bacteria and fermentation parameters in the digesta from five locations in the rumen of three cows at three time points were analysed. The PCR-denaturing gradient gel electrophoresis (PCR-DGGE) profiles were similar among digesta samples from five locations (95 center dot 4%) and three time points (93 center dot 4%) within cows; however, a lower similarity was observed for samples collected from different host animals (85 center dot 5%). Rumen pH and concentration of volatile fatty acids (VFA) were affected by time points of sampling relative to feeding. Conclusions: The detectable bacterial structure in the rumen is highly conserved among different locations and over time, while the quantity of individual bacterial species may change diurnally in response to the feeding. Significance and impact of the study: This study supplies the fundamental understanding of the microbial ecology in the rumen, which is essential for manipulation of ruminal microflora and subsequent improvement in animal production.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据